| Backyard Trails LLC | Trail Builders | Cedar Rapids | | 1 |
| Barr Bicycle & Fitness | Bike Shop | Des Moines | | 1 |
| Beaverdale Bicycles | Bike Shop | Des Moines | | 2 |
| Bennett's Bike And Fitness | Bike Shop | Mason City | | 1 |
| Bike Tech | Bike Shop | Cedar Falls | | 9 |
| Bike World (west) | Bike Shop | West Des Moines | | 1 |
| Bike World - Ames | Bike Shop | Ames | | 8 |
| Bike World - Urbandale | Bike Shop | Urbandale | | 3 |
| Bike World - West | Bike Shop | West Des Moines | | 4 |
| Broken Spoke, The | Bike Shop | Iowa City | | 2 |
| Cedar Valley Association for Soft Trails | Trail Association | Cedar Falls | | 5 |
| Central Iowa Trails Association | Trail Association | Des Moines | | 3 |
| Decorah Bicycles | Bike Shop | Decorah | | 4 |
| Decorah Human Powered Trails | Trail Association | Decorah | | 0 |
| Des Moines Bike Collective | Bike Shop | Des Moines | | 3 |
| Europa Cycle And Ski | Bike Shop | Cedar Falls | | 4 |
| Ezekiel's Wheel | Bike Shop | Ocheyedan | | 0 |
| Free Flight | Bike Shop | Dubuque | | 1 |
| Friends of Off-Road Cycling | Trail Association | | | 3 |
| Geoff's Bike And Ski | Bike Shop | Iowa City | | 3 |
| Hall Bicycle | Bike Shop | Cedar Rapids | | 1 |
| Healthy Habits | Bike Shop | Bettendorf | | 3 |
| Ichi Bike | Bike Shop | Des Moines | | 1 |
| Ingawanis Woodland Trails | Trail Association | Waverly | | 3 |
| Iowa Bike Company | Bike Shop | Pella | | 0 |
| Iowa Coalition of Off-Road Riders | Trail Association | Solon | | 1 |
| Irwin's Bike And Sport | Bike Shop | Johnston | | 2 |
| Kyle's Bikes | Bike Shop | Ankeny | | 5 |
| Letsche's Bike Shop | Bike Shop | Cherokee | | 0 |
| Linn Area Mountain Bike Association | Trail Association | Cedar Rapids | | 1 |
| Magellan Bike And Ski Repair | Bike Shop | Maquoketa | | 1 |
| Modern Bike | Bike Shop | Des Moines | | 1 |
| North Gorge Group | Trail Association | Burlington | | 0 |
| North Iowa Human Powered Trails | Trail Association | Mason City | | 0 |
| Northtowne Cycling And Fitness | Bike Shop | Cedar Rapids | | 3 |
| Raccoon River Valley Bicycle | Bike Shop | Perry | | 0 |
| Rasmussen Bike Shop | Bike Shop | West Des Moines | | 4 |
| Red Rock Area Mountain Bike Trails Association | Trail Association | Pella | | 0 |
| Riverside Cyclery | Bike Shop | Ottumwa | | 0 |
| Scheels - The Southern Hills Mall | Bike Shop | Sioux City | | 2 |
| Skunk River Cycles | Bike Shop | Ames | | 2 |
| Sugar Bottom Bikes | Bike Shop | North Liberty | | 3 |
| The Broken Spoke | Bike Shop | Iowa City | | 3 |
| Tri-State Mountain Bike Riders | Trail Association | Dubuque | | 4 |
| Vinton Off Road Cyclists | Trail Association | Vinton | | 0 |
| Wayne's Ski & Cycle | Bike Shop | Mason City | | 3 |
| World Of Bikes | Bike Shop | Iowa City | | 3 |